Int Conf AIDS 1996 Jul 7-12; 11:228 (abstract no. Tu.A.503) Levy JA, Mackewicz C, Barker E, Stranford S; Cancer Research Institute, University of California, San Francisco, CA, USA. Fax: 415-476-8365. E-mail: j.levy@itsa.ucsf.edu.
The alpha and beta chemokines have been shown to block replication of certain strains of HIV in peripheral blood CD4+ cells and established cell lines. However, the concentration of these products required for efficient suppression of virus is above the range normally found endogenously produced by CD8+ cells. Moreover, other cells can produce these chemokines including CD4+ cells. The ability of these chemokines to influence infection of CD4+ cells is currently under study. Physicochemical analyses, the kinetics of chemokine production and levels of chemokine secretion by lymphocytes indicate that these substances are not the CD8+ cell antiviral factor (CAF) that we have described. Moreover, CAF production correlates with an asymptomatic state while levels of chemokine production by CD8+ cells are similar among infected individuals manifesting different clinical stages. The antiviral activity of CD8+ cell fluids containing CAF likewise are not affected by antibodies to these alpha and beta chemokines that are below those shown to have antiviral activity in vitro. Finally, CAF production by herpesvirus saimiri-transformed CD8+ cell lines does not correlate with levels of beta chemokine production. The potential interaction of RANTES, MIP-1alpha, MIP-1beta and other chemokines with HIV is under further study.
Keywords: AEGIS, HIV Infections, Chemokines, RANTES, Macrophage Inflammatory Protein-1, Chemokines, CC, Virus Replication, HIV, HIV Core Protein p24, Antigens, CD8, Antigens, CD4, Herpesvirus 2, Saimiriine, In Vitro, virology, ICA11
