AEGiS-11IAC: Herpes-like virus (HHV-8) DNA sequences in various types of immunocompromised patients with Kaposi's sarcoma.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Herpes-like virus (HHV-8) DNA sequences in various types of immunocompromised patients with Kaposi's sarcoma.

Int Conf AIDS 1996 Jul 7-12; 11:8 (abstract no. We.A.167)
Friedman-Kien AE, Li JJ, Jensen P, Huang YQ; NYU Medical Center, Department of Microbiology, New York, NY, USA. Fax: 212-263-7933. E-mail: FRIEDA02@pop.mail.med.nyu.edu.

OBJECTIVE: To determine whether HHV-8 could be detected in KS lesions found in organ transplant recipients and other patients on immunosuppressive therapy.

METHODS: 14 tumor specimens were obtained from various patients with KS. Among them were 1 renal transplant recipient; 2 with rheumatoid arthritis who were on long term immunosuppressive therapy; 6 were classical KS (3 of these, females); and 5 were patients with AIDS-KS. 5 samples of hemangioma were used as controls. DNA was extracted from formalin-fixed-paraffin embedded biopsy sections. The quality of DNA was tested by PCR amplification for beta-globin. The PRC detection for HHV-8 was performed using a specific HHV-8 primer pair followed by Southern blot hybridizations using a 32P-ATP labeled probe. (table: see text)

CONCLUSION: These results indicate that HHV-8 was detected not only in AIDS-KS and CKS tumor biopsy specimens, but also in KS tissue from iatrogenically immunocompromised renal transplant recipients and patients with rheumatoid arthritis with KS. It seems that HHV-8 is associated with the occurrence of KS and this viral agent may play a role in the etiology and/or pathogenesis of KS seen in different epidemiologic populations.

Keywords: AEGIS, Herpesvirus 8, Human, Sarcoma, Kaposi, Acquired Immunodeficiency Syndrome, Blotting, Southern, Polymerase Chain Reaction, Immunosuppression, Kidney Transplantation, Paraffin Embedding, Liver Transplantation, Organ Transplantation, Heart Transplantation, China, Human, Female, transplantation, surgery, ICA11

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