AEGiS-11IAC: Multicenter quality control of plasma HIV-1 RNA quantitation by a standardized reverse-transcriptase PCR assay.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Multicenter quality control of plasma HIV-1 RNA quantitation by a standardized reverse-transcriptase PCR assay.

Int Conf AIDS 1996 Jul 7-12; 11:17 (abstract no. We.A.523)
Izopet J, Bargues G, Bocket-Mouton L, Brun-Vezinet F, Burgard M, Cottalorda J, Descamps D, Dussaix E, Krivine A, Fleury H, Pellegrin I, Poggi C, Profizi N, Rouzioux C, Seigneurin JM, Tamalet C, Puel J; Laboratoire de Virologie, CHU Purpan, Toulouse, France. Fax: (33) 61 77 25 42.

OBJECTIVE: We recently assessed the intra-assay, inter-assay, and inter-lot reproducibilities of a standardized RT-PCR assay (Izopet et al., J. Virol. Methods, in press). This study used the same panel to assess the inter-laboratory reproducibility.

METHODS: A panel consisting of 10 coded plasma samples was obtained from 9 HIV-1 infected volunteers and 1 uninfected subject. The blood samples were collected into sodium citrate Cell Preparation Tubes (Becton Dickinson), processed within 2 h of venipuncture, aliquoted and stored at -80. Sets of aliquots were shipped frozen on dry ice to 11 french virology laboratories for testing. All tests were done using the Amplicor HIV-1 Monitor assay by trained operators.

RESULTS: 1/ All labs reported that the negative sample had no detectable HIV-1 RNA, demonstrating the absence of amplicon contamination. 2/ The pooled results from all 11 labs on the 9 positive samples were: (table: see text)

CONCLUSIONS: 1/ Overall, the performance of the laboratories was good. 2/ As expected, the inter-laboratory reproducibility (mean 0.23 log) of the Monitor assay was greater than the inter-assay reproducibility (mean 0.10 log) on a single site. 3/ A quality assurance program and the use of common control materials should help laboratories assaying plasma HIV-1 RNA to identify systematic differences.

Keywords: AEGIS, Reverse Transcriptase Polymerase Chain Reaction, HIV-1, Plasma, HIV-1 Reverse Transcriptase, Laboratories, virology, ICA11KWDaegis,reversetranscriptasepolymerasechainreaction,hiv-1,plasma,hiv-1reversetranscriptase,laboratories,virology,ica11

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WeA523

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