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11th International AIDS ConferenceVancouver, British Columbia — July 7-12, 1996 |
Table of Contents - Published Abstracts, Track A: Basic ScienceMo = Monday, Tu = Tuesday, We = Wednesday, Th = Thursday, Lb = Late-Breaker Cite as: Int Conf AIDS. 1996 Jul 7-12;11:Abstract No. xx |
| Pub.A.1001 | HIV-1 nucleocapsid protein (NCp7) specifically directs initiation of (-) DNA synthesis in cell-free reverse transcriptase assays. Int Conf AIDS 1996 Jul 7-12; 11:438 (abstract no. Pub.A.1001) Li X, Kleiman L, Parniak MA, Wainberg MA; McGill University AIDS Centre-Jewish General Hospital, Montreal, Canada. Fax: (514) 340-7537. Objective: To determine the physiological relevance of NCp in reverse transcription. Methods: Reactions were performed using recombinant HIV RT in the presence of NCp. Results: In the presence of tRNALys.3, NCp7 was found to stimulate synthesis of minus-strand strong-stop DNA [(-) ss DNA], consistent with previous repo |
| Pub.A.1002 | Synergistic antagonism of HIV reverse transcriptase activity by monoclonal antibodies in the presence of NNRTI's. Int Conf AIDS 1996 Jul 7-12; 11:438 (abstract no. Pub.A.1002) Gu Z, Parniak MA, Wainberg MA; McGill University AIDS Centre-Jewish General Hospital, Montreal, Canada. Fax: (514) 340-7537. Objective: To assess the ability of a monoclonal antibody termed 1E8 to impact on HIV reverse transcriptase (RT) activity in the presence of both nucleoside triphosphate (ddNTP) inhibitors of RT and non-nucleoside RT inhibitors (NNRTI). Methods: Cell-free RT assays were performed with all 4 dNTPs plus 1E8 MAb in the pr |
| Pub.A.1003 | Endogenous HIV reverse transcriptase assays are more sensitive than other methods in determinations of HIV drug resistance. Int Conf AIDS 1996 Jul 7-12; 11:438 (abstract no. Pub.A.1003) Quan Y, Gu Z, Wainberg MA; McGill University AIDS Centre-Jewish General Hospital, Montreal, Canada. Fax: (514) 340-7537. Objective: To determine the utility of endogenous HIV reverse transcriptase (RT) assays in assays of drug resistance. Methods: Fully endogenous RT asays were performed using either wild-type (wt) or drug-resistant mutated viruses. These studies utilize the viral genome as template and are performed with lysed virions i |
| Pub.A.1004 | Heat shock specific amplification of HIV provirus. Int Conf AIDS 1996 Jul 7-12; 11:438 (abstract no. Pub.A.1004) Angelika V, Kozlova AV, Abelian AV, Yolov AA, Kornilayeva GV, Karamov EV; A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia. Fax: 939-31-81. Objective: To evaluate viral DNA load in acutely HIV-infected cells under a heat shock (HS, HS-cells). Methods: HS of HIV RF infected H9 lymphoblastoid line cells at 41.5 C for 1 h followed by propagation in presence of selected azidothyimidne concentration in order to prevent reinfection. HIV provirus copy number has |
| Pub.A.1005 | Comprehensive community-based sample collection and testing for the assessment of HIV and STD/GTI prevalence in rural Uganda. Int Conf AIDS 1996 Jul 7-12; 11:438 (abstract no. Pub.A.1005) McNairn D, Paxton L, Wabwire-Mangen F, Wawer M, Quinn T, Sewankambo NK, Gaydos C; Makerere Univ. Kampala, Uganda. Objective: We assessed the utility of several tests not previously used extensively in a community-based setting to determine the prevalence of HIV and other STD/GTIs (Genital tract infection) in a rural population of Uganda . Methods: Between Nov 1994 and June 1995, we established a cohort consisting of all consenting |
| Pub.A.1006 | IgA anti-HIV-1 antibody in cerebrospinal fluid and serum samples from adult HIV-infected patients. Int Conf AIDS 1996 Jul 7-12; 11:438 (abstract no. Pub.A.1006) Ueda M, Yamashiro R, Oliveira CA, Sanches RF, Paz RB, Palhares MC; STD/AIDS Reference & Training Centre-Secretary of Health of Sao Paulo State, Sao Paulo, Brazil. Fax: 55-11-853.3505. Objective: To evaluate the occurrence of specific IgA antibody (Ab) to HIV-1 in paired CSF and serum samples from HIV-infected patients (px) with CNS alterations or neurologic complaints. Methods: From 371 adult male and female HIV-positive px clinically assessed by CDC AIDS case-definition criteria, 437 paired CSF/ser |
| Pub.A.1007 | Evaluation of particle agglutination test (serodia) for determination of single and dual HIV-1/2 infections in Bombay. Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1007) Gharpure H, Godse C, Hira S, Kurimura T; Department of Infectious Diseases, MGM Medical College, New Bombay, India. Fax: 91-22-444 0378. Objective: To evaluate efficiency of particle agglutination test (Serodia) to differentiate single and dual HIV-1/2 infections. Methods: One hundred and four consecutive patients attending HIV/AIDS referral outpatient service at MGM Hospital in New Bombay were recruited in the study. Serum samples were tested by ELISA |
| Pub.A.1008 | A rapid flow cytometric method detects reduced T cell proliferative responses to phytohaemagglutinin (PHA) in HIV-infected subjects. Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1008) Trickett AE, Cameron B, Kwan YL; Southpath, St George Hospital, Sydney, NSW, Australia. Fax: +61-2-350-3942. Objective: Using a rapid, whole blood, flow cytometric method this study aimed to compare the mitogen-induced T lymphocyte proliferative response of HIV-infected patients with that of healthy uninfected controls. Methods: Blood samples from 12 uninfected male subjects (mean CD4/microliter = 923, range 790 - 1188) and 1 |
| Pub.A.1009 | In vivo antiviral activity of didanosine evaluated in the model of macaques infected by a pathogenic isolate of SIVmac251. Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1009) Franck M, Cheret A, Caufour P, Neildez O, Theodoro F, Vaslin B, Clayette P, Le Grand R, Dormontd; Service de Neurovirologie, Commissariat a l'Energie Atomique, DRM, DSV, SSA, France. Fax: (33-1) 46 54 77 26. Objectives: To evaluate the antiviral efficacy of didanosine during the acute experimental infection of macaques with a pathogenic isolate of simian immunodeficiency virus (SIVmac251). Materials and methods: two groups of 4 and 2 cynomolgus macaques were treated by three daily subcutaneous injections of didanosine with |
| Pub.A.1010 | Preferential recognition of V3 African sequences and relation with disease progression among Cuban HIV-positive individuals. Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1010) Lopez-Campistrous Ana, Reyes O, Garay H, Diaz H, Duarte C; Center for Genetic Engineering and Biotechnology, Havana, Cuba. Objective: To analyse the serological response in HIV-1 Cuban seropositive individuals against five synthetic V3 loop peptides, and to correlate it with the clinical stage of these subjects. Methods: 35-meric (spanning the whole V3 loop) and 15-meric (spanning the tip of the loop) peptides were prepared. Plasmas from 6 |
| Pub.A.1011 | Correlation between neutralizing properties and genomic composition of Mexican HIV-1 isolates. Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1011) Barquet A, Gudino JC, Soler C; Unidad de Investigacion en Retrovirus Humanos. Instituto de Investigaciones Biomedicas, UNAM and Instituto Nacional de Diagnostico y Referencia Epidemiologicos, SSA. Mexico. Fax: 3413264. E-mail: csoler@servidor.unam.mx. Objectives: To correlate data from neutralization assays and the V3sequences in 7 strains of HIV-1. METHODS. Neutralization assays were carried out with 43 sera against 500 TCID50 of 7 different viral strains (MEXP2; MEXP4; MEXA3; MEXP42; MEXP42m; MEXP42p and IllB/LAV) using SupT1 as target cells. A 320 bp dsDNA fragme |
| Pub.A.1012 | HIV-1 suppressive activity from transformed CD8 cells efficiently inhibits virus activation. Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1012) Mosoian A, Piwoz J, Klotman ME; Mt. Sinai School of Medicine, New York, NY, USA. The existence of soluble antiviral activity in CD8+ lymphocytes (CAF) was first reported several years ago by Levy and co-workers. To further study this activity, we have recently isolated a number of transformed (with Herpesvirus Saimiri, HVS), purified CD8+ cells from a cohort of pediatric long-term survivors and rap |
| Pub.A.1013 | Some patterns of AIDS pathogenesis in Ukraine. Int Conf AIDS 1996 Jul 7-12; 11:440 (abstract no. Pub.A.1013) Marichev IL, Antonenko SV, Scherbinskaya AM, Kruglov YV, Barbasheva YV, Protsap EI, Grin EV, Frolov AF; Institute of Epidemiology & Infective Diseases, Health Ministry of Ukraine, Kyiv, Ukraine. Fax: (044) 277-2411. Objective: In 1992-1994 the increase of AIDS patients was rather low (25-40 cases/year); this marker became 3 times higher in 1995 due mostly to intravenous drug abusers. Taking into consideration the unfavourable social and economical changes in Ukraine due to Chernobyl explosions well as immunopopulational changes an |
| Pub.A.1014 | HIV-infection effect on immune system cells genome. Int Conf AIDS 1996 Jul 7-12; 11:440 (abstract no. Pub.A.1014) Antonenko SV, Barbasheva YV, Shcherbinska AM; Research Institute of Epidemiology & Infectious Diseases, Kiev, Ukraine. Fax: 380(044)277-24-00. Objective: To study interdependence of genetic stability and functional activity of immunocompetent cells at different HIV infection stages. Methods: The levels of spontaneous DNA repair synthesis, quantity of single-stranded breaks in DNA peripheric lymphocytes, sensitivity to damage influences, ability to restorate U |
| Pub.A.1015 | Replicative and cytopathic characteristic of AZT-resistant HIV-1 strains. Int Conf AIDS 1996 Jul 7-12; 11:440 (abstract no. Pub.A.1015) Selimova LM, Pokrovsky VV; The D.I. Ivanovsky Institute of Virology, Moscow, Russia. Fax: 095-1902867. E-mail: nick@nasel.msk.ru. Objective: To revealed the replicative and the cytopathic capacity of AZT-resistant HIV-1 strains isolated from individuals after AZT therapy. Methods: HIV-1 strains MS-1974 and MS-1978 were grown in Jurkat-tat, MT-4, CEM, and U-937 cells lines. Viral activity was determined by WB, syncytium-inducing capacity and IF. T |
| Pub.A.1016 | Regulation of HIV-1 replication by mutants of the IFN inducible protein kinase PKR. Int Conf AIDS 1996 Jul 7-12; 11:440 (abstract no. Pub.A.1016) Koromilas AE, Nagai K, Li S, Hiscott J, Sonenberg N, Wainberg M; McGill University, Montreal, Canada. Fax: (514) 340-7576. E-mail: mdak@musica.mcgill.ca. Objective: PKR is an interferon (IFN) inducible serine-threonine kinase which regulates protein synthesis. Activation of PKR by viruses results in phosphorylation of the translation initiation factor eIF-2, a modification that causes inhibition of protein synthesis. HIV-1 infection results in a significant decrease in |
| Pub.A.1017 | Sequencing method for genetic analysis of HIV-1 in Thailand. Int Conf AIDS 1996 Jul 7-12; 11:440 (abstract no. Pub.A.1017) Isarangkura Na Ayuthaya P, Daungchandra S, Saipradit N, Sriwanthana B, Naganawa S, Auwanit W, Bumisawasdi J, Jayavasu C, Miyamura K, Honda M, Yamazaki M; Department of Medical Science, Ministry of Public Health, Thailand. Fax: 662-5899869. E-mail: watimm@health.moph.go.th. Objective: To establish an appropriate sequencing method of genetic analysis for characterization of HIV-1 prevalence in Thailand using autosequencer. Methods: Genomic DNA were extracted from PBMCs of 20 HIV-1 infected patients (10 IDUs, 10 heterosexuals), were amplified by nested PCR for HIV V3 domain using a nested P |
| Pub.A.1018 | Genetic characteristic of HIV-1 in Thailand. Int Conf AIDS 1996 Jul 7-12; 11:440 (abstract no. Pub.A.1018) Auwanit W, Bumisawasdi J, Isarangkura Na Ayuthaya P, Daungchandra S, Saipradit N, Sriwanthana B, Naganawa S, Jayavasu C, Miyamura K, Honda M, Yamazaki M; Department of Medical Science, Ministry of Public Health, Thailand. Fax: 662-5899869. E-mail: watimm@health.moph.go.th. Objective: To characterize field HIV-1 in Thailand genetically and discuss the various factors involved in better understanding the character of HIV to plan and initiate such trials. Methods: Blood samples from 352 individuals seropositive for HIV-1 were collected from intra-venous drug users (IVDU), and sexually trans |
| Pub.A.1019 | Molecular epidemiology of HIV-1 infection in the former USSR. Int Conf AIDS 1996 Jul 7-12; 11:441 (abstract no. Pub.A.1019) Bobkov AF, Cheingsong-Popov R, Selimova L, Kazennova E, Karasyova N, Ladnaya N, Pokrovsky V, Weber J; The D.I. Ivanovsky Institute of Virology, Moscow, Russia. Fax: 095-190-2873. E-mail: NICK@nasel.msk.ru. Objective: To characterize the HIV-1 genetic subtypes circulating in different risk groups in the former Soviet Union republics. Methods: PBMC DNA samples derived from HIV-1 infected individuals from different risk groups in the former USSR were analysed by heteroduplex mobility assay (HMA) and env gene sequencing. Res |
| Pub.A.1020 | Molecular epidemiology of HIV/AIDS in St. Petersburg, Russia. Int Conf AIDS 1996 Jul 7-12; 11:441 (abstract no. Pub.A.1020) Kozlov A, Emeljanov A, Malykh A, Barabitskaya O, Verevotchkin S, Farzadegan H, Reitz MC; Biomedical Center for AIDS, Cancer and Related Problems, Research Institute of Pure Biochemicals, St. Petersburg, Russia. Fax: 7-(812)-230-4948. E-mail: HIVAIDS@mod.hpb.spb.ru. Objectives: In our previous works (Kozlov et al., 1993,1994) we have shown that Russia is presently experiencing an early stage of HIV/AIDS epidemic. It was interesting to study molecular epidemiology of this early stage of epidemic. Methods: Using the approach of nested PCR with multiple primers we were able to amplif |
| Pub.A.1021 | Characterization of HIV-1 dual infection in patients of Rio de Janeiro. Int Conf AIDS 1996 Jul 7-12; 11:441 (abstract no. Pub.A.1021) Filho J, Pinto M, Costa L, Brinfeiro R, Pieniazek D, Ryfield M, Shechter M, Tanuri A, Carvalho M; Cornell Univ. Medical College, Division of International Medecine, Lab, New York, NY, USA. To analyse the HIV-1 protease and p24 gag genes in potentially dual infected HIV-positive patients. Methods: Viral protease and p24gag genes were amplified by nested PCR, The amplified products of protease were digested with AluI,HinfI, BclI and ScaI. p24gag products were didested with DraI, AluI and HaeIII. Res |
| Pub.A.1022 | Cell genome stabilization by yeast RNA-tilorone complex in in vitro HIV-infected cells. Int Conf AIDS 1996 Jul 7-12; 11:441 (abstract no. Pub.A.1022) Karpov AV, Antonenko SV, Barbasheva YV, Spivak NY; D. Zabolotny Institute of Microbiology & Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine. Fax: 380 (044)266-23-79. Objective: We have earlier detected interferonogenic and immunomodulating effects of yeast RNA-tilorone molecular complexes (MC). We have also showed inhibition of in vitro HIV replication due to this preparation Optimization of conditions necessary for MC fawarable action is of great interest. Methods: Investigation o |
| Pub.A.1023 | Recruitment for an HIV seroincidence study in Brazil: alternative approaches and lessons learned. Int Conf AIDS 1996 Jul 7-12; 11:441 (abstract no. Pub.A.1023) Santos E, Lago RF, Silva SB, Costa RB, Schechter M, Harrison LH; Universidade Federal do Rio de Janeiro, Brazil. Difficulties in the recruitment of participants for Projeto Praca Onze, a HIVNET-sponsored HIV seroincidence study in Rio de Janeiro, Brazil . Project: Projeto Praca Onze is a study to evaluate HIV seroincidence and risk factors for HIV transmission among 1,000 men who have sex with men attending 5 anonymous HIV |
| Pub.A.1024 | The development of virus-like-particles (VLPs) as candidate vaccines based on the prevalent strains in HIV epidemic area of Southwest China. Int Conf AIDS 1996 Jul 7-12; 11:441 (abstract no. Pub.A.1024) Shao Y, Guan Y, Zhao Q, Zeng Y, Chang J, Wagner R, Wolf H; Institute of Virology, Chineses Academy of Preventive Medicine, Beijing, China. Fax: 3532053. E-mail: yshao@net.tsinghua.edu.cn. Objective: To develop a candidate vaccines of VLPs based on the prevalent HIV strains in a epidemic area bordering Myanmar in the Southwest China . Methods: HIV-1 env and gag genes from local HIV-1 infected injecting drug users (IDU) were amplified and sequenced. The prevalent HIV strains of this region were selected a |
| Pub.A.1025 | Kamizol: a new drug for HIV-infections and secondary immunodeficiency treatment. Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1025) Spivak NY, Karpov AV, Antonenko SV, Barbasheva YV; D. Zabolotny Institute of Microbiology & Virology,National Academy of Sciences of Ukraine, Kiev, Ukraine. Fax: 380(044) 266-23-79. Objective: To determine the anti-HIV and immunomodulation activities of kamizol, a new derivative from the group of phenylimidazothyazole. Methods: Kamizol was obtained by original synthesis using tetramyzole and alkylic reagents. Its anti-HIV activity was studied using infected MT-4 H-9, CEM and U-937 cells lines. The |
| Pub.A.1026 | Dysregulated cytokine kinetics prior to AIDS. Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1026) Salazar-Gonzalez JF, Martinez-Maza1 O, Nishanian PG, Aziz N, Grosser S, Taylor JM, Detels R, Fahey JL; UCLA School of Medicine, Dept. of Microbiology & Immunology, Los Angeles, CA. Objective: To examine the kinetics of production of serum levels of selected cytokines and surrogate activation markers in the 4 years preceding AIDS diagnosis, in HIV seropositive (HIV+) subjects manifesting an accelerated decline in CD4 numbers. Methods: Ten HIV+ individuals (cases) that demonstrate a rapid decline i |
| Pub.A.1027 | Interleukin(IL)-12 induces IL-10 expression in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals. Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1027) Daftarian PM, Kumar A, Diaz-Mitoma F; Dept. of Micro. & Immuno., University of Ottawa, Ottawa, Canada. Fax: 613-738-4819. Objective: To evaluate the effect of IL-12 on IL-10 production of PBMC from HIV-infected individuals. Methods: PBMC from twenty seven HIV+ and five HIV- individuals were studied for the in vitro effect of IL-12 on IL-10 production. PBMC were cocultured with recall antigens or PHA or media alone with or without IL-12. I |
| Pub.A.1028 | A clinico-microbiological profile of HIV illness in Calcutta, India. Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1028) Banerjee KL, Mukherjee P, Chakrabarti T, Maiti P, Pal N, Chakraborty MS; Department of Medicine, Calcutta Medical College, Calcutta, India. Fax: 91-033-554-5741. Objectives: To determine the clinical abnormalities and microbial infections in AIDS related maladies. Methods: A total of 42 cases of HIV illness have been followed since Sept. 1988 in the eastern part of India , with a view to ascertain the nature of clinical manifestations and the microbial infections causing diseas |
| Pub.A.1029 | Interaction of HIV-*1 and human herpes simplex virus (HSV)in mixed infection. Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1029) Barbasheva YV, Antonenko SV, Gryn YV, Shcherbinska AM; Research Institute of Epidemiology & Infectious Diseases, Kiev, Ukraine. Fax: 044-277-24-00. Objective: To study some events concerning HIV-1 and HSV interaction in in vitro and in vivo mixed infection. Methods: The titers of HIV and HSV antibody have been determined in samples of 44 HIV-positive patients. The levels of HIV-1 (p24) and HSV antigens in cell supernatants have been made using ELISA. Some biologic |
| Pub.A.1030 | Prevention of recurrent genital herpes in HIV-infected males with a dual combination therapy: human leukocyte interferon-alpha in cream and oral zidovudine. Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1030) Tanweer AS, Seyed AA, Seyed HA; Department of Clinical Physiology, Malmo University Hospital, University of Lund, Sweden. Fax: INT.+ 40-336254. Objective: The aim of this open study was to determine the frequency of recurrent genital herpes infections in HIV-infected patients, and to evaluate suppression, tolerance and safety of a dual combination therapy comprising human leukocyte interferon-alpha(2 x 106 IU/g) in cream and oral Zidovudine (250 mg). |
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