AEGiS-12IAC: Prevalence of deletion in the beta-chemokines receptors (Δ32 Ccr5) sequency codificator in blood donors in Sao Paulo city (Brazil).

12th International AIDS Conference

Geneva, Switzerland - June 28-July 3, 1998

Prevalence of deletion in the beta-chemokines receptors (Δ32 Ccr5) sequency codificator in blood donors in Sao Paulo city (Brazil).

Int Conf AIDS 1998 Jun 28-Jul 3; 12:7 (abstract no. 11123)

Silva MC, Rossini MA, Sabino EC
Instituto Adolfo Lutz, Sao Paulo, Brazil.

OBJECTIVE: To investigate the prevalence of 32pb deletion (Δ32 CCR5) in the beta-Chemokines receptors sequence in blood donnors in Sao Paulo city/Brazil.

DESIGN: Prevalence Study.

METHODS: Amplification by PCR with "primers" that flank the coding region for the 32pb mutation in beta-Chemokines receptors CCR5 (Δ32 CCR5), using isolated DNA from mononuclears periferic blood cells from 200 blood donors of the Sao Paulo city/Brasil. The differentiation from genotypes was analized on a 2% agarose gel.

RESULTS: From a total of 200 samples analized, 187 (93.5%) presented wild type genotype (wt/wt), without deletion for the CCR5 receptor; in 13 samples (6.5%) we find the heterozygous genotype for the deletion (wt/Δ32). We didn't find the homozygous genotype (Δ32/Δ32) for the deletion on the CCR5 receptor in any of the studied samples.

CONCLUSIONS: The absence of the homozygous deletion character (Δ32/A 32) for the CCR5 receptor in Sao Paulo city/Brazil population, can be due to the small size but can also be related with a large racial interbreeding existing in this country.

Keywords: AEGIS, Prevalence, Blood Donors, Receptors, CCR5, Chemokines, CC, Genotype, Cities, Polymerase Chain Reaction, Mutation, Brazil, epidemiology, genetics, ICA12
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