AEGiS-12IAC: Heat shock modulation of HIV-1 infection.

12th International AIDS Conference


Geneva, Switzerland - June 28-July 3, 1998

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Heat shock modulation of HIV-1 infection.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:8 (abstract no. 11129)

Karamov EV, Yudin AN, Evgen'ev MB, Abelian AV, Kornelayeva GV, Kozlova AV, Ulmasov KhA
Ivanovski Institute of Virology, Moscow, Russia.

OBJECTIVES: To study participation of heat shock proteins (HSPs) in HIV-cell intimate relationships.

METHODS: H9 lymphoblastoid cell line, infected with HIV-1 HTLV-IIIRF NIH 1983. Western blot with monoclonal antibodies against different families of human HSPs. Heat shock induction (heat treatment at 41.5 degrees C for 60 min). Quantitative DNA-PCR on HIV provirus. ELISA for anti-HSP antibodies.

RESULTS: H9 cells infected with HIV-1RF, produce additional HSPs 75, 40 and 32 kD. First one resembles to GRP. Heat shock induction increases number of HIV provirus copies. Amplification has two phases: 1. Immediate; it takes 3-5 h after heat treatment (3 times difference) and 2. Late; after 24-36 h (up to 6 times difference). Second is probably due to preferable proliferation of HIV-infected cells. Some HIV-infected persons (up to 25%) have serological reactivity towards HSP 60.

CONCLUSIONS: HIV-1 infection changes profile of HSPs synthesized in the cell which leads to higher resistance to alteration and induces anti-HSP antibody production.

Keywords: AEGIS, Heat, HIV Infections, HIV-1, Heat-Shock Proteins, Blotting, Western, Heat-Shock Response, Deltaretrovirus, Proviruses, HIV Seropositivity, Human, ICA12KWDaegis,heat,hivinfections,hiv-1,heat-shockproteins,blotting,western,heat-shockresponse,deltaretrovirus,proviruses,hivseropositivity,human,ica12
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11129

Copyright © 1998 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.