AEGiS-12IAC: The role of the cytoskeleton of HIV-infected cells as the transportation system of viral structural proteins.

12th International AIDS Conference

Geneva, Switzerland - June 28-July 3, 1998

The role of the cytoskeleton of HIV-infected cells as the transportation system of viral structural proteins.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:9 (abstract no. 11137)

Takahashi I, Takama M, Ushijima K, Nonomura Y, Hoshino H
Lab. EM. Teikyo Univ. Sch. Med., Japan.

OBJECTIVES: To clarify the role of cytoskeleton of HIV-infected cells that would play an important part to transport viral structural proteins during viral producing processes.

METHODS: Lymphocyte strains (Molt-4/HTLV-III B, TALL-1/KB-1) and monocyte strains (U937/HTLV-III B) were used and fixed for the immuno-electron microscopy. Eeach thin sections were immuno-stained with 1st Abs (anti-gag MoAbs: p17/p24, anti-env MoAbs: gp41/gp120, anti-cytoskeleton MoAbs: actin/intermediate filament(IF) etc.) and with colloidal golds as 2nd Abs.

RESULTS: Each strains revealed common features, i.e., the viral structural proteins were synthesized on the surfaces of rough endoplasmic reticulum etc. and then via long fibrous structures those were transported to just under the plasma membrane of infected cell. These fibrous structures were identified as IF that were located from around nuclei to plasma membranes. On the other hand contrary to expectations, actin filaments were depolimerized that were no more able to observe as usual filamentous structures.

CONCLUSION: From these results, it was revealed that among cytoskeletons just intermediate filament played a role to transport viral structural poteins in HIV-infected cells.

Keywords: AEGIS, HIV, HIV Infections, Viral Structural Proteins, HIV Seropositivity, HIV Antibodies, HIV Antigens, HIV Long-Term Survivors, Anti-HIV Agents, Animal, pathogenicity, immunology, ICA12
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