AEGiS-12IAC: Variability of the V3-loop amino acids of HIV-1 group O strains in Cameroun.

12th International AIDS Conference


Geneva, Switzerland - June 28-July 3, 1998

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Variability of the V3-loop amino acids of HIV-1 group O strains in Cameroun.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:16 (abstract no. 11171)

Gurtler L, Zekeng L, Eberle J, Brunn AV, Kaptue L
Pettenkofer Institut, University of Munchen, Germany.

OBJECTIVE: To further characterize the diversity of HIV-1 group O strains and to build up their evolutionary tree compared to group M sequences.

METHODS: Group O infected patients in Cameroun were identified by a screening algorithm with a HIV-1 + 2 + O Elisa on the first level, and an anti-HIV-1 competitive and gp41-O Elisa on the second level. From blood lymphocytes DNA was isolated and in part of the samples virus isolated. Using special (cam5env and cam3env) and additional primers polymerase chain reaction yielded amplificates that were sequenced and their nucleic acid and amino acid sequences established. Sequences were aligned and compared by the Treecon programme.

RESULTS: The crown motif of the Ant70 isolate GPMAWY was found most frequent in 24/45 (53%) strains, followed by the GPLAWY motif in 9/45 (20%) isolates. The GPMRWR motif of the MVP5180 was not found in a further isolate, but the GPLSWY in 3, GPLAIY in 1, GPMARY in 1, GPMASL in 1, GPMSWY in 1, GPMGRY in 1, SPLAWY in 1 and DPMAWY in 2 (linked) strains. The crown motif did not predict the clustering found by the computer analysis using the whole C2-V3 region. Three to 5 clusters were found, headed by the Ant70, MVP5180 and the Ham112 isolate. The clusters were identical for most isolates when a further part of the p24-gag-gene was analyzed. Using amplificates from the conserved gp41 N-terminal region revealed much less divergence of the strains, but predominant sites of amino acid exchanges.

CONCLUSION: Depending on the region of the genome studied group O viruses show a high degree of diversity which is similar to that of group M viruses. The evolutionary tree of group O shows until now no predominant strains.

Keywords: AEGIS, HIV-1, HIV-2, Genes, gag, Amino Acid Sequence, Polymerase Chain Reaction, Enzyme-Linked Immunosorbent Assay, Human, genetics, ICA12KWDaegis,hiv-1,hiv-2,genes,gag,aminoacidsequence,polymerasechainreaction,enzyme-linkedimmunosorbentassay,human,genetics,ica12
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