AEGiS-12IAC: Distribution of HIV-1 subtypes among HIV-1 positive tuberculosis (TB) patients in the interior of Cote d'Ivoire.

12th International AIDS Conference

Geneva, Switzerland - June 28-July 3, 1998

Distribution of HIV-1 subtypes among HIV-1 positive tuberculosis (TB) patients in the interior of Cote d'Ivoire.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:18-9 (abstract no. 11182)

Nkengasong J, Abouya L, Kalou M, Coulibaly D, Pau C, Borget MY, Boateng E, Sassan-Morokro M, Respess R, Coulibaly R, Wiktor SZ, Greenburg AE, Rayfield M
Project Retro-CI, Abidjan, Cote d'Ivoire.

OBJECTIVE: To describe the distribution of genetic subtypes of HIV-1 strains in different regions of Cote d'Ivoire.

METHODS: Plasma and peripheral blood mononuclear cells (PBMC) were obtained from HIV-1-seropositive TB patients in all the TB outpatient clinics in the interior of Cote d'Ivoire including Abengourou (n = 27), Bouake (n = 49), Daloa (n = 24), Korhogo (n = 31), Gagnoa (n = 12), and Man (n = 29). Plasma samples were tested by a V3-loop peptide enzyme immunoassay (PEIA) that uses consensus synthetic V3-loop peptides derived from subtype A strains from Abidjan, and other HIV-1 subtypes (B, C, D, E, F, G) within group M viruses, and one HIV-1 group O peptide. PBMC were tested by a restriction fragment length polymorphism (RFLP) assay that involves a sequential endonuclease digestion of a 297-base pair PCR fragment using AluI, BclI, and ScaI to segregate the HIV-1 viral strains into distinct subtypes. DNA sequencing of the protease or env genes was performed on all samples discordant in the two assays as well as a random sample of the concordant samples.

RESULTS: Of the 171 specimens, three were PCR-negative and 168 PCR-positive. Of the PCR-positive samples 147 (88%) were classified as subtype A by RFLP and 21 were untypeable. Of the 147 RFLP subtype A samples, 137 (93%) were subtyped A by PELA, three, two, and five were classified as subtype C, D, and F by PEIA, respectively. All 21 untypeable samples were subtype A by both PEIA and by protease sequencing. Sequencing of the env gene classified the 10 PEIA non-subtype A samples as three As, two Ds and five Gs. Sequencing of a subset of 10 concordant samples confirmed them all as subtype A. Therefore, 161 (91%) of the specimens were env subtype A, two env subtype D and five env subtype G.

CONCLUSIONS: As in Abidjan, the vast majority of HIV-1 infected TB patients throughout the Cote d'Ivoire interior are infected with subtype A. A combination of PEIA and RFLP assays can rapidly and accurately subtype a majority of HIV-1 samples thereby greatly reducing the need to perform DNA sequencing.

Keywords: AEGIS, HIV-1, Genes, env, Tuberculosis, HIV Seropositivity, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Immunoenzyme Techniques, Cote d'Ivoire, Human, Male, genetics, ICA12
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