AEGiS-12IAC: Comparative analysis of genetic diversity among seropositive populations in Kenya.

12th International AIDS Conference


Geneva, Switzerland - June 28-July 3, 1998

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Comparative analysis of genetic diversity among seropositive populations in Kenya.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:20 (abstract no. 11188)

Songok EM, Ichimura H, Tukei PM, Kakimoto K, Orege P, Sakagami N, Kurimura T
Virus Research Centre, Nairobi, Kenya.

OBJECTIVE: To determine the HIV-1 subtypes in various risk groups and different locations in Kenya.

DESIGN: Cross sectional molecular epidemiological study.

METHODS: Blood samples were collected in Western Kenya (Kisumu) and Nairobi from antenatal clinic attendees (368), patients with STDs (326), HIV+ children born to HIV+ mothers (34) and blood donors (68). Serological testing was done by Particle agglutination test and ELISA. After DNA isolation (DNAzol), direct sequencing was carried on the C2-V3 region of proviral DNA. Sequence analysis was done by UPMGA and neighbour joining methods.

RESULTS: Western Kenya had the highest prevalence rate of 30.5% and 45.4% in antenatal clinic attendees and STD populations respectively. Nairobi had prevalences of 16.4% and 37.5%. Of a total of 95 isolates successfully sequenced, HIV subtype A was the most prevalent, (80%), followed by sutype D (9.4%), subtype C (7.3%) and subtype G (1%). Two isolates could not be assigned to any of the known subtypes. Despite the significant differences in the prevalence there was no difference in subtypes. However, there was a tendency of HIV subtype D to cluster within the STD risk group but this association was statistically insignificant.

CONCLUSION: Our data confirms the dominance of HIV subtype A in most infected asymptomatic populations in Kenya and report the first presence of subtype G. Difference between the prevalences of HIV in various locations and populations may not be attributed to differences in HIV clades.

Keywords: AEGIS, HIV-1, Variation (Genetics), HIV Infections, HIV, Prevalence, HIV Envelope Protein gp120, Kenya, HIV Core Protein p24, Population, HIV Envelope Protein gp41, Cross-Sectional Studies, Blood Donors, Child, Human, genetics, immunology, ICA12KWDaegis,hiv-1,variation(genetics),hivinfections,hiv,prevalence,hivenvelopeproteingp120,kenya,hivcoreproteinp24,population,hivenvelopeproteingp41,cross-sectionalstudies,blooddonors,child,human,genetics,immunology,ica12
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