AEGiS-12IAC: Determination of genetic subtypes of HIV-1 from Korean patients with advanced AIDS.

12th International AIDS Conference

Geneva, Switzerland - June 28-July 3, 1998

Determination of genetic subtypes of HIV-1 from Korean patients with advanced AIDS.

Int Conf AIDS 1998 Jun 28-Jul 3; 12:22 (abstract no. 11201)

Choe KW, Oh MD, Kim JW, Shin DH, Park SW, Shin HS, Park KH
Dept. Internal Medicine, Seoul National University Hospital, South Korea.

BACKGROUND: To determine the subtypes of HIV-1 in Korean patients with advanced AIDS, we analyzed the nucleotide sequences of HIV proviral DNA and constructed a phylogenetic tree.

METHODS: Peripheral blood mononuclear cells (PBMCs) were collected from 19 patients with AIDS who were referred from all over the country. DNA was extracted and proviral V3 env sequence was amplified by polymerase chain reaction (PCR). DNA sequences of PCR product were determined by cycle sequencer with fluorescence labeled dye terminators and aligned with a set of reference sequences. A phylogenetic tree was constructed by neighbor joining method.

RESULTS: Enrolled patients were in the advanced stage of AIDS with CD4 counts ranging from 2 to 40 (median 17). Sequencing analyses of the PCR products (ca 330 bp) revealed eighteen cases (95%) of subtype B and one case (5%) of subtype A. The tetrameric motifs at the tip of the V3 loop were comprised of GPGR, GPGS (six cases, 32%, respectively), GPGK, GPGG (two cases, 11%, respectively), and GPGQ (one case, 5%).

CONCLUSIONS: Subtype B of HIV-1 was predominant and only one case was of different clade (subtype A) in advanced AIDS patients in Korea.

Keywords: AEGIS, HIV-1, Acquired Immunodeficiency Syndrome, Base Sequence, Polymerase Chain Reaction, CD4 Lymphocyte Count, Korea, Human, genetics, ICA12
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