AEGiS-12IAC: Requirement of each glycan in the gp120 for maintaining the infectivity of simian immunodeficiency virus (SIV).

12th International AIDS Conference

Geneva, Switzerland - June 28-July 3, 1998

Requirement of each glycan in the gp120 for maintaining the infectivity of simian immunodeficiency virus (SIV).

Int Conf AIDS 1998 Jun 28-Jul 3; 12:24 (abstract no. 11212)

Nagai Y, Ohgimoto S, Shioda T, Mori K, Nkayama EE, Hu H
Dept. of Viral Infection, Univ. of Tokyo, Japan.

OBJECTIVES: To know the role of each N-linked oligosaccharide in infectivity of SIVmac239.

METHODS: Twenty-three mutant SIVmac239 constructs each lacking one of the 23 N-linked oligosaccharide chains in the gp120 envelope protein were generated according to the standard in vitro mutagenesis method. Mutant viruses were recovered by transfection of each construct to SW480 cells followed by cocultivation with MT4 cells.

RESULTS: Compared with the wild-type parental virus for replication capability in MT4 cells, the 23 mutants were either equally replicating, severely impaired or faster replicating. The respective three different types of mutations did not distribute randomly but clustered to particular regions in the primary amino acid sequence of the gp120. The faster replicating mutants exhibited a higher fusogenic activity.

CONCLUSION: For maintaining SIV infectivity, each N-linked glycan of gp120 is either dispensable, essential or down-regulating, depending on its location.

Keywords: AEGIS, SIV, Polysaccharides, Virus Replication, Amino Acid Sequence, Oligosaccharides, In Vitro, pathogenicity, virology, ICA12
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