AEGiS-13IAC: Detection of drug resistance in HIV-1 subtype E subjects using LIPA assay.

13th International AIDS Conference

Durban, South Africa - July 9-July 14, 2000

Detection of drug resistance in HIV-1 subtype E subjects using LIPA assay.

Int Conf AIDS 2000 Jul 9-14; 13:20 (abstract no.. LbOr15)

Louisirirotchanakul S, Suttment R, Pirunla-Ong T, Wasi C
Siriraj Hospital, Mahidol University, Bangkok, Thailand. Fax: +66 2 418 41 48.

BACKGROUND: In Thailand, subtype E has been found to be the majority of HIV-1 infection via heterosexual. Intervention of vertical transmission of HIV-1 using short course (Bangkok regimen) of AZT to HIV-1 infected pregnant women during the last trimester and delivery has been proposed. Thus, the essential of HIV-1 genotypic resistance in infected subjects should be explored in Thai infected subjects.

OBJECTIVE: To survey the emergence of drug resistance to anti-retroviral drugs in HIV-1 subtype E subjects.

METHODS: A total of 34 plasma collected from HIV-1 infected subjects (10 pregnant women and 24 subjects who had drug experiences) were assessed for genotypic resistance mutations using line probe assay (LIPA) compared to pol sequencing. In addition, 24 plasma from HIV-1 infected patients in naive and drug experience were also analysed.

RESULTS: Only 47% (16 out of 34) from both groups could be amplified with RT PCR primers probably due to low viral load. In spite of the absence of wild type at codon 41, single mutation at codon R70.V74 and F215 from 4 subjects either on AZT or combination of AZT/ddI therapy were not observed in LIPA pattern. While the remainders seemed too sensitive to AZT. The emergence of drug resistance strain on RT at codon 41 and 214/215 was detected in one subject.

CONCLUSIONS: HIV drug resistance testing prior to anti-retroviral drug prescription has been proposed in HIV-1 infected subjects especially HIV-1 infected women during pregnancy. Although LIPA with the 48 probes covering at least 6 different regions has been approached to be a rapid genetic testing for B variants, the absence of reactivity on codon 41, 69/70 an 74 has been reported in HIV-1 subtype E infection. These polymorphism with undetectable reactivity codon in LIPA need to be developed for HIV-1 subtype E polymorphisms.

Keywords: AEGIS, HIV-1, HIV Infections, Zidovudine, HIV-1 Reverse Transcriptase, Viral Load, Drug Resistance, Disease Transmission, Vertical, Codon, Mutation, Didanosine, Thailand, Human, Female, Pregnancy, transmission, genetics, AIDS
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