AEGiS-13IAC: T-lymphocyte subsets as markers for discrimination between HIV--infected and uninfected children.

13th International AIDS Conference

Durban, South Africa - July 9-July 14, 2000

T-lymphocyte subsets as markers for discrimination between HIV--infected and uninfected children.

Int Conf AIDS 2000 Jul 9-14; 13:35 (abstract no.. LbPeA7014)

Kositanont U, Wasi C, Sutthoent R, Foongladda S, Manprapar N, Chearskul S, Bowonokiratikachorn P, Chimabutra K, Yamanisai K
Seriraj Hospital, Mahidol University, Bangkok, Thailand. Fax: +66 2 418 41 48.

BACKGROUND: Diagnosis of HIV-infection children born to HIV- infected mothers is determined by detection of proviral HIV- I DNA or HIV antibody persistence beyond 18 months of age. The objective of this study was to determine whether T-lymphocyte subsets as markers for discrimination between HIN-infected and uninfected children during the first year of life.

METHODS: One hundred and forty-seven infants born to HIV-scropostive mothers were enrolled. The infants were followed prospectively from 1-2 months to 18 months or more. The blood specimens from the infants were tested for T-lymphocyte subsets, anti-HIV and proviral HIV-1 DNA in blood mononuclear cells using polymerase chain reaction (PCR).

RESULTS: The vertical transmission of HIV-1 subtype E was 27 of 147 cases (18.4%). By analysis of variance, no difference between sexes was observed in all testings. The CD4 count of <750 cells/mm³ and CD4% of <15 were undetectable in HIV-uninfected children, except in the age-group 1-3 months. During 4 to 12 months old, there was an strong association between CD4 count of <750 and CD4% of <1 5 and HIV-infection in children (x2 test.p<0.001). In addition, the results showed a strong relationship between CD8 count of ≥ 2250 (OR 13.36. p<0.001), CD8% 35 (OR 39.24, p<0.00 1) and CD4/CD8 ratio of <0.2(OR undefined p<0.00 1).

CONCLUSIONS: During 4-6 months of age, CD4 count of <750, CD4% of <15. CD8 count of ≥ 2250, CD8% of >35, and CD4/CD8 ratio of <0.2 were used as markers for discrimination between HIV-infected and uninfected children born to HIV-seropositive mothers.

Keywords: AEGIS, T-Lymphocyte Subsets, HIV Infections, HIV Seropositivity, CD4 Lymphocyte Count, Disease Transmission, Vertical, HIV-1, Biological Markers, Antigens, CD8, Antigens, CD4, HIV Antibodies, Child, Human, Infant, pathogenicity, transmission, immunology, AIDS
000709
LbPeA7014