AEGiS-13IAC: The five newly derived HIV-1 group N strains cluster together in the HIV/-1/SIV cpz lineage.

13th International AIDS Conference


Durban, South Africa - July 9-July 14, 2000

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The five newly derived HIV-1 group N strains cluster together in the HIV/-1/SIV cpz lineage.

Int Conf AIDS 2000 Jul 9-14; 13:(abstract no. MoOrA166)

Souquiere S, Roques P, Ayouba A, Robertson DL, Apetrei C, Muelller-Trutwin M, Barre-Sinoussi F, Nerrienet E, Simon F
S. Souquiere, Laboratoire de Retrovirologie, Centre International de Recherches Mejicales, Franceville BP769, Gabon, Tel.: +241 677 092, Fax: +241 677 295, E-mail: souquiere@cirmf.sci.ga

BACKGROUND: Studies have shown a close relationship between SIVcpz from common chimpanzees (Pan. troglotydes troglotydes ) and HIV-1 group N. However, only one HIV-1 N (YBF30) have so far been fully characterized, and a second group N strain (YBF105) partially sequenced in pol. The objectives of present study was to evaluate group N epidemiology and to better define the HIV-1/SIV cpz relationship.

METHODS: 6500 HIV-1 positives samples collected in Cameroon (1997-99) were tested using group M, O and N-specific V3 peptides EIA. A 150 pb fragment of integrase was amplified for three new group N samples designated YBF106, 115 and 116. Plasma RT-PCR remained negative for the fourth case (YBF117). In addition YBF106 was fully sequenced. YBF115 and 116 sequences are in progress (env gp41 being sequenced).

RESULTS: In serological assay 97% samples reacted with the group M peptide, 2% with the group O peptide and four samples (0.1%) with group N peptide. Phylogenetic analysis showed a monophyletic clustering of all five group N strains in integrase and env analyses confirmed this clustering. The HIV-1 group N and SIVcpz strains form a monophyletic cluster. This cluster is particularly important (100% of bootstrap values) between group N and SIVcpz isolated from wild caught chimps from Cameroon.

CONCLUSION: These new sequences confirm a primate-human cross-species transmission and the circulation of group N strains in humans. The low prevalence in juvenile chimps points to the need for epidemiological studies of adult primates populations to find the missing links in the origin(s) of HIV groups M and O

Keywords: AEGIS, SIV, HIV-1, HIV Infections, Pan troglodytes, HIV Seropositivity, Cameroon, Epidemiologic Studies, Animal, Adult, HumanKWDaegis,siv,hiv-1,hivinfections,pantroglodytes,hivseropositivity,cameroon,epidemiologicstudies,animal,adult,human
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MoOrA166

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