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13th International AIDS ConferenceDurban, South Africa - July 9-July 14, 2000 |
Int Conf AIDS 2000 Jul 9-14; 13:(abstract no. TuOrA412)
Roques P, Souquiere S, Damond F, Couturier E, Farfara I, Dormont D, Simon F, Fleury H, Brun-Vezinet F
P. Roques, CEA, Service de Neurovirologie, B.P.6, Fontenay-aux-Roses, France, Tel.: +33 1 46 54 76 74, Fax: +33 1 46 54 77 26, E-mail: roques@dsvidf.cea.fr
BACKGROUND: To study the HIV non B strains from the "Observatoire des souches de VIH-1 en France" to assess recombinations in a population of known date of infection. 2) To evaluate the impact of the recombination of viruses on epidemiological survey in terms of prevalence of anti-HIV drug resistant mutations in such a population an reliability of viral load evaluation in infected patients.
METHODS: : Between September 1996 to 1998, 2168 adult patients with positive HIV-1 Western blot were included in an ongoing national surveillance of HIV-1 subtypes. The year of HIV infection was estimated in a subgroup for which the dates of the last negative and first positive HIV test were available. HMA studies have shown that all subtypes A to H are present in France. Subtypes were assessed by sequencing env and gag regions. Phylogenetic analysis were performed (clustalW, PHILIP). Recombination points were checked using Diversity plot.
RESULTS: Serological (SSEIA) and HMA subtyping show a 16% prevalence of non-B subtypes (295 patients) mainly from Africa or with African partners. HMA studies have shown that all subtypes A to H are present in France. . Among the 552 HIV-1 (+) patients with an estimated date of infection, 483 were infected with B subtype and 69 with non-B subtype. We analysed 63 types B and 63 non-B of known infection date. Sequencing from gag and RT-pol gene shown that (40%) of sub-types A were from the A/G ibNG sub-division. Amongst the analysed sequence only one present mosaic sequence with env-B and non-B gag sequences while 7 recombinants were gag-B/env-non-B. Two viruses are complexes mosaic with unexpected pol sequences.
CONCLUSIONS: This study points out the diversity of HIV strains in France and the fast introduction of new subtypes. 13% of non-B strains and less than 2% of env-B viruses are recombinants showing that B infected population remains phylogenetically stable.
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