AEGiS-13IAC: Assessment of cytokines on CD4+T lymphocytes in HIV-1 infected patients and the impact of HAART on that expression.

13th International AIDS Conference


Durban, South Africa - July 9-July 14, 2000

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Assessment of cytokines on CD4+T lymphocytes in HIV-1 infected patients and the impact of HAART on that expression.

Int Conf AIDS 2000 Jul 9-14; 13:(abstract no. WeOrB489)

Skokanova V, Stankova M, Spala J, Beneda M, Rozsypal H, Machala L
V. Skokanova, AIDS Center Univ. Hospital Bulovka, Institute of Hygiene, Dittrichova 17, 12801 Praha 2, Czech Republic, Tel.: +420 2 249 149 66

BACKGROUND: To assess and characterise changes in expression of IL-4 and IFN-g on CD4+ T lymphocytes during HIV-1 infection in patients without therapy, and after initiation of HAART.

METHODS: We followed 13 HIV-positive patients, who were treated at least 6 months by HAART and 4 HIV-positive patients without therapy. In the control group we selected 7 HIV-negative healthy persons.Flow cytometric analysis was employed for direct detection of intracellular cytokines visualized by fluorochrome labeled monoclonal antibodies.

RESULTS: In HIV patients without therapy minimal expression of both examined cytokines was noted (IL-4: 0.25% without, 0.42% after stimulation, IFN-g: 0.19% without, 0.59% after stimulation), compared to normal healthy volunteers (IL-4: 0.63% without, 3, 75% after stimulation, IFN-g: 0,46% without, 13,37% after stimulation). Group of HAART- treated patients, where treatment lasted at least 6 months, showed significant elevation of both cytokines expression (IL-4: 1,19% without, 4,08% after stimulation, IFN-g: 0,72%without, 5,65% after stimulation).

CONCLUSION: Our results demonstrate significant elevated expression of Th-1 (IFN-g) and Th-2 (IL-4) cytokines in a group of HIV patients treated by HAART at least 6 months, when compare to the HIV patients without therapy. IL-4 production was slightly higher in patients with HAART than in HIV negative control group.Long-lasting expression of cytokines during HAART is probably welcome sign of the immune system reconstitution. The study is supported by the Grant IGA MZ ER 3950-3.

Keywords: AEGIS, Antiretroviral Therapy, Highly Active, HIV-1, HIV Infections, Cytokines, HIV Seropositivity, Interleukin-4, HumanKWDaegis,antiretroviraltherapy,highlyactive,hiv-1,hivinfections,cytokines,hivseropositivity,interleukin-4,human
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WeOrB489

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