AEGiS-14IAC: Frequency of sdf-1 utr801-a in distinct brazilian populations.

14th International AIDS Conference

Barcelona, Spain - July 7-12, 2002

Frequency of sdf-1 utr801-a in distinct brazilian populations.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. A10007)

Grimaldi RS, Acosta AX, Argollo MC, Galvao-Castro B
Laboratorio Avancado de Saude Publica - Centro de Pesquisas Goncalo Moniz - Fundacao Oswaldo Cruz, Salvador, Brazil

BACKGROUND: SDF-1, human stromal cell-derived factor, is a member of the CXC intercrine family. This chemokine has an important role in lymphocyte activation and traffic. SDF-1 has the CXCR4 as its receptor. Previous studies have demonstrated that the CXCR4 functions as a coreceptor for the fusion/entry/infection mechanism by the X4 HIV-1. A single nucleotide polymorphism (SNP) in the SDF-1 gene described as a G-to-A transition at the position 801 in the 3-prime-UTR (801-A), seems to be related with a delay in the onset of AIDS when in homozygosis. The frequency of the 801-A was prevalent in Caucasians, Hispanics, and Asians, being rare in African Americans. Our objective was to identify the allelic frequency of this SNP in three distinct ethnic groups of Brazil.

METHODS: Thus, the genomic DNA extraction from 180 whole blood individuals was obtained using DNAzol commercial kit, than submitted to PCR followed by enzymatic digestion using MspI.

RESULTS: In 46 individuals of Salvador, a city in the northeast region, with a population of 80% black or racially mixed (Portuguese and Africans), we found the allelic frequency of 0.20. In 42 blood donors from Joinville, located in the south region, with a population mainly of German's descendents, the frequency found was 0.30. In the Waiampi and Tiriyo Brazilian tribes (descendents from ancient Mongolian populations and an isolated population in the north region) 92 samples were studied (46 of each tribe), whose allelic frequency was 0.17 and 0.25, respectively.

CONCLUSIONS: In fact, our results are in accordance with the literature data, that shows a homogeneous distribution of the 801-A allele, being more prevalent in Caucasians. Despite the predominance of black people in Salvador population, the results revealed a higher value of the mutant allele than expected, probably due to a Caucasian mixture. We showed part of the results of an extending research that studies the genetics factors involved with resistance to AIDS.

Keywords: AEGIS, Chemokines, CXC, Gene Frequency, HIV-1, Alleles, Acquired Immunodeficiency Syndrome, European Continental Ancestry Group, Population, African Continental Ancestry Group, Ethnic Groups, Polymerase Chain Reaction, Brazil, stromal cell-derived factor-1alpha, Human, epidemiology, genetics

020707
A10007