AEGiS-14IAC: Polymorphism of CCR5, CCR2 and CCR5 Promoter on HIV-1infection in a cohort from Sao Paulo, Brazil.

14th International AIDS Conference

Barcelona, Spain - July 7-12, 2002

Polymorphism of CCR5, CCR2 and CCR5 Promoter on HIV-1infection in a cohort from Sao Paulo, Brazil.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no.. A10008)

Rigato PO, Hong MA, Casseb JS, Araujo RN, Araujo PF, Bueno AG, Ueda M, Orii NM, Duarte AJ
School of Medicine of University of Sao Paulo, Sao Paulo, Brazil

BACKGROUND: HIV-1 binds to CD4 associated to coreceptors to entry into the cells. The coreceptors usage can determine viral tropism and disease progression. Once few studies have been done among Brazilian, we aimed to genotype CCR5, SDF-1, CCR2b and CCR5 Promoter (CCR5-Pro) and to evaluate the effect of their polymorphism on disease progression.

METHODS: Genotyping was done by PCR. For SDF-1, CCR2b and CCR5-Pro a complementary RFLP was done on PCR product using Msp-1, BsaB1 and BSP1268 enzyme. The amplicons were evaluated on a 2-3% agarose gel.

RESULTS: Among 154 HIV-1-infected patients followed at the Out Patient ADER302 Clinic of Clinical Hospital of School of Medicine of USP, we observed 8,4% and 25,3% of CCR5-~32 and SDF-1 3'A, respectively, and 4,5% of SDF-1'A/'A. Also, 25% were heterozygous (HET) and 0,8%, homozygous mutant (MUT) for CCR2b-64I allele (n=120). For CCR5-Pro (n=48), 66,7% were HET for CCR5 59029 G/A allele. In general, 86% had at least 1 mutation. There wasn't difference in the polymorphism distribution by gender, age, CD4 or viremia. Patients WT for both CCR5 and SDF-1 were more likely to have AIDS diagnosis at the study entry than others, although they both have shown similar disease progression during the following up.

CONCLUSION: Our data are very similar to those previously reported in Brazil, Europe, Asia and Africa. Genetic polymorphism of coreceptors seems to play some role on the disease progression as have been shown previously in Caucasian.

Keywords: AEGIS, HIV, Polymorphism (Genetics), HIV Infections, Promoter Regions (Genetics), HIV-1, Alleles, Disease Progression, Receptors, HIV, Chemokines, CXC, HIV Seropositivity, Genotype, Polymorphism, Restriction Fragment Length, HIV Long-Term Survivors, Polymerase Chain Reaction, Mutation, Brazil, Asia, Africa, Europe, stromal cell-derived factor-1alpha, Human, genetics

020707
A10008