AEGiS-14IAC: HLA-B, HLA-DRB1, TNF-α and ccr5 ~ 32 allele and haplotype frequencies and susceptibility to HIV infection in Mexicans.

14th International AIDS Conference

Barcelona, Spain - July 7-12, 2002

HLA-B, HLA-DRB1, TNF-α and ccr5 ~ 32 allele and haplotype frequencies and susceptibility to HIV infection in Mexicans.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. A10060)

Zuniga JA, Quiroz-Morales VS, Alvarado-Osuna DA, Vargas-Alarcon G, Granados J, Reyes-Teran G
Unit of Infectious Diseases for Immunocompromised Patient, Instituto Nacional de Enfermedades Respiratorias., Mexico, Mexico

BACKGROUND: Several MHC loci have been involved with resistance to HIV infection or with different patterns of HIV-related disease progression. However, the alleles associated differ among several populations.

OBJECTIVE: To determine the MHC alleles and CCR5Δ32 gene deletion in a Mexican Mestizo (MM) HIV-infected cohort.

METHODS: Seventy-seven HIV-infected MM subjects and 99 ethnically-similar uninfected individuals, as a contrasting group, were studied. HLA-B, DRB1, TNF-α -308 alleles and CCR5Δ32 deletion were analyzed by PCR procedures. The differences were evaluated by non parametric statistics using the EPIINFO statistical program. The p values were corrected by the Bonferroni method.

RESULTS: We observed an increased frequency of HLA-B*39 and B*15 antigens and a significant decreased frequency of B*49 in the HIV cohort (pC=0.04; OR=0.16; 95 % CI=0.05-1.25). The DRB1 alleles were equally distributed among both groups. We also observed a significant increase in the frequency of TNF2 allele in the HIV-infected group (pC=0.02; OR= 2.95; 95 % CI= 1.03-8.82). The genotype TNF1/TNF1 was less common in patients as compared to controls (pC=0.03; OR= 0.35; 95 % CI= 0.11-1.04). The HLA-[B*39-DRB1*04-TNF1],[B*35-DRB1*04-TNF1],[B*40-DRB1*04-TNF1] and [B*35-DRB1*08-TNF1] haplotypes, were common in both groups. We observed a non significant increase of B*39 haplotypes in HIV-infected group. The distribution of CCR5Δ32 gene deletion in HIV cohort (1.2%) and in the uninfected group was uncommon.

CONCLUSION: These preliminary results indicate an increased frequency of HLA-B*39, B*15 antigens and TNF2 allele, and a decreased frequency of HLA-B*49 in the MM HIV-infected cohort. Further studies are needed to confirm if our results are associated with the susceptibility (HLA-B*39, B*15 and TNF2 alleles) or resistance (HLA-B*49) to HIV infection. Our results also suggest that CCR5Δ32 does not play an important role in HIV infection in Mexicans.

Keywords: AEGIS, HLA-B Antigens, HLA-DR Antigens, Haplotypes, Alleles, HIV Infections, Disease Susceptibility, Tumor Necrosis Factor, Hispanic Americans, Genotype, Polymerase Chain Reaction, HLA-DRB1, Human, immunology, genetics

020707
A10060