14th International AIDS Conference


Barcelona, Spain - July 7-12, 2002

DonateNow
Print this article

Three years long follow up of immunological reconstitution in HIV-1 infected patients treated with HAART.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. A10080)

Skokanova V, Spala J, Machala L, Stankova M, Rozsypal H
Dept. of Immunology, Regional Hygienic Station, Prague, Czech Republic

BACKGROUND: to analyze the immunological reconstitution in 10 HIV-1 positive patients six, eighteen and thirty months after initiation of HAART.

METHODS: We assessed and characterized changes in expression of IL-4 and IFN-γ on CD4+T lymphocytes and number of T cells during HIV-1 infection in patients before HAART and six, eight and thirty months after initiation of HAART. Flow cytometric analysis was used for direct detection of intracellular cytokines and for detection T lymphocytes.

RESULTS: In 10 HIV-1 infected patients treated six months with HAART was observed statistically significant elevation of expression both intracellular cytokines IL-4 (4,39%, p=0,045) and IFN-γ (6,09%, p=0,008) after stimulation on CD4+T lymphocytes when compared with the untreated HIV+ patients (IL-4: 0,42%, IFN-γ: 0,59%). After eighteen months of treatment was the expression of both cytokines lower (IL-4: 4,32%, IFN-γ: 3,72%) and after thirty months again higher (IL-4: 7,12%, IFN-γ: 4,33%) but differences were not statistically significant. After initiation of HAART was observed elevation of CD4+T cells, naïve and memory CD4+ and CD8+ cells and decrease of CD8+T cells, activated CD3/HLA-DR cells and plasma viral load. This status outlasts also eighteen and thirty months after start of therapy except elevation of plasma viral load after thirty month on therapy. All these changes are not statistically significant.

CONCLUSIONS: Our results demonstrate that six months after initiation of HAART appears significant improvement of the status of immune system - the restoration of immune functions and the correction of T-cell subsets abnormalities. This improvement outlasts still eighteen and thirty months after initiation of HAART. The study was supported by the grant IGA MZ CR 6303-3.

Keywords: AEGIS, Antiretroviral Therapy, Highly Active, HIV-1, Viral Load, HIV Infections, CD8-Positive T-Lymphocytes, T-Lymphocyte Subsets, Interleukin-4, Antigens, CD8, Antigens, CD4, Antigens, CD3, Human, immunologyKWDaegis,antiretroviraltherapy,highlyactive,hiv-1,viralload,hivinfections,cd8-positivet-lymphocytes,t-lymphocytesubsets,interleukin-4,antigens,cd8,antigens,cd4,antigens,cd3,human,immunology

020707
A10080

Copyright © 2002 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.