AEGiS-14IAC: Prevalence of HIV-1 resistant to antiretroviral drugs in Thailand.

14th International AIDS Conference


Barcelona, Spain - July 7-12, 2002

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Prevalence of HIV-1 resistant to antiretroviral drugs in Thailand.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. B10180)

Jenwitheesuk E, Watitpun C, Pongthanapisith V, Vibhagool A, Sungkanuparph S, Chantratita W
Ramathibodi Hospital, Bangkok, Thailand

BACKGROUND: Prolonged treatment with antiretroviral drugs results in the selection of HIV-1 variants with mutations conferring resistance to nucleoside and non-nucleoside reverse transcriptase inhibitors (NRTI and NNRTI) or to protease inhibitors (PI).

METHODS: This study report on the frequency of genetic mutation associated with drug resistance in antiretroviral-treated patients from Thailand. Resistance testing was performed on 88 individuals. Automated sequencing was used to genotype the reverse transcriptase (RT) and protease regions of virus isolated from patients' plasma.

RESULTS: A high prevalence of PI and RT genotypic variants, associated with high-level resistance to antiretroviral drugs, was observed (PI = 12%, RT = 70%). The PI mutations, V82A/F, L90M and G48V, were found in 8, 7 and 3% of cases, respectively; whereas for RT, primary mutations at positions T215Y/F (zidovudine), M184 (lamivudine), K103N (multi-NNRTI) and Q151M (multi-NRTI) were present in 45, 27, 12 and 3% of subjects, respectively. The mutations conferring low-level resistance to lopinavir plus ritonavir, foscanet, amprenavir and tenofovir were found in 9%, 7%, 5%, and 3% of cases respectively. The mutations associated with highly resistance to amprenavir were also found in 6% of cases. In addition, as found in the other previous studies in Thailand, by phylogenetic analysis, primarily subtype CRF01_AE viruses were identified in 82 (93%) of cases, whereas 6 (7%) of 88 were subtype B.

CONCLUSIONS: Our results indicate that, the frequency of HIV-1 primary genotypic resistance implying adjustment of the antiretroviral drug regimen in Thailand is high. In addition, the high prevalence of drug resistance HIV-1 may limit in future the effectiveness of antiretroviral programs in Thailand.

Keywords: AEGIS, HIV-1, Prevalence, Reverse Transcriptase Inhibitors, HIV-1 Reverse Transcriptase, Thailand, Genotype, Mutation, RNA-Directed DNA Polymerase, Human, epidemiology, geneticsKWDaegis,hiv-1,prevalence,reversetranscriptaseinhibitors,hiv-1reversetranscriptase,thailand,genotype,mutation,rna-directeddnapolymerase,human,epidemiology,genetics

020707
B10180

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