AEGiS-14IAC: High prevalence of Intersubtype recombination in the pol gene HIV-1 in the South region of Brazil.

14th International AIDS Conference


Barcelona, Spain - July 7-12, 2002

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High prevalence of Intersubtype recombination in the pol gene HIV-1 in the South region of Brazil.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. C10732)

Silva WP, Santos C, Pereira AN, Filho DS, Diaz RS
University Federal of Sao Paulo, Sao Paulo, Brazil

BACKGROUND: HIV-1 Clades B, F, and C cocirculate in Brazil, and the majority of infected individuals harbors B viruses. Therefore, we, analyzed the clade profile of HIV-1 in long term infected individuals from Rio Grande do Sul, a State sited at the south region of Brazil.

METHODS: pol sequences of 74 consecutive patients failing HAART were analyzed. Resistance related codons at the reverse transcriptase (RT) and protease (PT) regions of pol were described as well as the clade profile.

RESULTS: Phylogenetic analyzes showed that 37 (50%) individuals were infected by clade B viruses, 3 (4%) by F, and 2 (2.7%) by C viruses. 43.3% of isolated viruses were recombinants: 12.2% were B-B/C (PT-RT respectively), and we also found F-B, F-F/B, C-C/B, B/F-B, F/B-F, C-B, C/F-C, B/F-F, F/B-F/B, B/F-B/C, B/C-B, F/B-B/C/F (PT-RT) in lower prevalences (less than 4%). The transitions from clade B to clade C genome at the RT region in the patients harboring B-B/C viruses were at the same genomic region. In contrast to previous reports, we were not able to find any differences on the prevalence of resistance related mutations between B and non-B strains.

CONCLUSIONS: We found a high prevalence of intersubtype recombination within the pol gene, and a high prevalence of B-B/C viruses with the same break point at the RT. The impact of the pol polymorphisms in non-B viruses may be of importance to ARV response.

Keywords: AEGIS, Genes, pol, HIV-1, Prevalence, HIV-1 Reverse Transcriptase, Recombination, Genetic, Antiretroviral Therapy, Highly Active, Mutation, RNA-Directed DNA Polymerase, Brazil, Human, epidemiology, genetics, virologyKWDaegis,genes,pol,hiv-1,prevalence,hiv-1reversetranscriptase,recombination,genetic,antiretroviraltherapy,highlyactive,mutation,rna-directeddnapolymerase,brazil,human,epidemiology,genetics,virology

020707
C10732

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