AEGiS-14IAC: Myeloid and plasmacytoid circulating dendritic cell numbers are already decreased in the first nine days after rectal SIV infection in rhesus monkeys.

14th International AIDS Conference


Barcelona, Spain - July 7-12, 2002

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Myeloid and plasmacytoid circulating dendritic cell numbers are already decreased in the first nine days after rectal SIV infection in rhesus monkeys.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. LbPeA9001)

Petitprez K, Kahi S, Jacques S, Andrieu M, Pretet JL, Hosmalin A, Butor C
ANRS, Paris, France

BACKGROUND: Dendritic cells (DC) are absolutely required to activate naïve T cells and they induce crucial early innate immune responses. Two major populations are found in the blood: myeloid CD11c+ DC, which secrete IL-12, and plasmacytoid CD123 (IL-3-R)+ DC, which secrete type I interferons, strong antiviral cytokines. We found that circulating DC numbers were dramatically decreased in HIV patients during primary infection (26 to 57 days post infection) (Pacanowski, Blood, 2001 ; 9:3016). Here we quantify DC in the very first days of rectal SIV infection in rhesus macaques.

METHODS:16 rhesus monkeys infected by the rectal route with 10 or 100 AID50 SIVmac251 were compared with 10 healthy animals. DC populations were quantified in blood samples from 16 hours to 9 days post infection (p.i.) using rare event analysis by flow cytometry.

RESULTS: The proportion of myeloid DC among peripheral blood mononuclear cells was decreased significantly as early as 24 hours p.i. in the SIV infected monkeys compared to the healthy animals (p=0.02). The proportion of plasmacytoid DC was also decreased between 16 and 48 hours p.i. (p=0.05).

CONCLUSION: We demonstrate for the first time that peripheral blood DC are decreased as early as 24 hours post rectal SIV infection, a good animal model for sexual transmission of HIV.

Keywords: AEGIS, SIV, Macaca mulatta, Dendritic Cells, Interleukin-12, HIV, Receptors, Interleukin-3, HIV Infections, Antigens, CD11c, Flow Cytometry, T-Lymphocytes, Rectum, human interleukin 3 receptor alpha subunit, Animal, Human, immunologyKWDaegis,siv,macacamulatta,dendriticcells,interleukin-12,hiv,receptors,interleukin-3,hivinfections,antigens,cd11c,flowcytometry,t-lymphocytes,rectum,humaninterleukin3receptoralphasubunit,animal,human,immunology

020707
LbPeA9001

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