AEGiS-14IAC: Use of dried blood spots for diagnosis and viral quantification in perinatal HIV-1 infection in Thailand.

14th International AIDS Conference

Barcelona, Spain - July 7-12, 2002

Use of dried blood spots for diagnosis and viral quantification in perinatal HIV-1 infection in Thailand.

Int Conf AIDS 2002 Jul 7-12; 14:(abstract no. WeOrB1339)

Chaowanachan T, Chokephaibulkit K, Chotpitayasunondh T, Wasinrapee P, Teeraratkul A, Jetsawang B, Neeyapun K, Simonds RJ, Young NL
Thai MOPH-US Centers for Disease Control and Prevention Collaboration, Nonthaburi, Thailand

BACKGROUND: To validate the use of field-site collected filter paper dried blood spot (DBS) samples for HIV-1 diagnosis and viral load (VL) monitoring of infants born to HIV-infected women in Thailand, where subtype E predominates.

METHODS: 442 paired DBS and whole blood samples in EDTA (blood) were collected from 2-month old infants born to HIV-infected women. All blood samples were tested using the modified Roche Amplicor HIV-1 DNA PCR test kit version v.1.0 (reference assay). Paired DBS for the 34 DNA PCR positive blood samples, and the first 100 DNA PCR negative blood samples were tested with the Roche Amplicor HIV-1 DNA PCR v.1.5 kit because the v.1.0 missed ~15% of non-B subtype DBS samples. Paired DBS and EDTA plasma samples were additionally tested using the qualitative Organon Teknika NucliSens RNA NASBA kit. The 34 positive blood samples also had EDTA plasma (reference assay) and DBS VL assays performed using the Roche Amplicor Monitor v.1.5 RNA PCR kit, with DBS results hematocrit-corrected. DBS samples were silica-gel extracted for both RNA assays. Reproducibility VL assays were set-up using 5 prepared DBS samples (VL range 1,626-35,245 copies/mL), which were stored at 37?, 25?, -20? and -37? C, and retested at 1, 3, 6, 9 and 12 months.

RESULTS: This table summarizes the

RESULTS: [table: see text] DBS VL results were reproducible within 0.5 log of baseline at all 4 temperatures for DBS with >10,000 copies/mL through 3 months.

CONCLUSIONS: Roche Amplicor DNA v.1.5 DBS, and NucliSens RNA plasma/DBS results were qualitatively comparable to the reference Amplicor DNA v.1.0 blood results. Roche Monitor RNA v.1.5 DBS VL results were quantitatively comparable to the reference Monitor RNA v.1.5 plasma VL results. DBS are reliable and stable for HIV-1 DNA/RNA detection and VL load monitoring.

Keywords: AEGIS, HIV-1, Viral Load, HIV Infections, HIV Seropositivity, Polymerase Chain Reaction, HIV, Exanthema, HIV Core Protein p24, HIV-1 Reverse Transcriptase, CD4 Lymphocyte Count, HIV Seronegativity, Thailand, Human, Female, Infant, diagnosis, blood, virology, utilization, immunology

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WeOrB1339