AEGiS-15IAC: A cis-regulatory element in HIV-1 env can co-operate with CTE to enhance Gag expression.

15th International AIDS Conference

Bangkok, Thailand - July 11-16, 2004

A cis-regulatory element in HIV-1 env can co-operate with CTE to enhance Gag expression.

Int Conf AIDS 2004 Jul 11-16; 15:(abstract no. TuOrA1134)

Auewarakul P, Suptawiwat O
Mahidol University, Bangkok, Thailand

BACKGROUND: We have previously described a cis enhancer sequence (CES) in the gp41 region of HIV-1 env downstream to RRE, which could co-operate with Rev and RRE to enhance Gag and Env expression.

METHODS: We tested whether the CES could function in CTE-dependent expression system by replacing RRE with CTE in expression contructs with and without CES.

RESULTS: CES could up-regulate CTE-dependent Gag expression. The up-regulation of Gag proteins was accompanied by the increase of intron-containing HIV-1 RNA in the cytoplasm but not in the nucleus.

CONCLUSIONS: Our data indicated that CES might activate nuclear export of intron-containing RNA via both Rev/RRE- and CTE-mediated pathways.

Keywords: AEGIS, Genes, env, Genes, gag, HIV-1, Gene Products, gag, Immediate-Early Proteins, Introns, CIS protein, genetics

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TuOrA1134