16th International AIDS Conference Toronto, Canada - August 13 - 18, 2006

EVALUATION OF TWO VOLUMETRIC FLOW CYTOMETERS FOR THE QUANTITATION OF CD4+ T CELLS IN THAI HIV-1-INFECTED PATIENTS

Int Conf AIDS. 2006 Aug 13-18;16 Abstract No. TuAb0205

Y. Phuang-ngern¹, V. Pobkeeree², S. Lerdwana¹, E. Noulsri¹, N. Onlamoon¹, S. Nookhai², K. Sukapirom¹, K. Fox³, J. Tappero², K. Pattanapanyasat^1
1 Faculty of Medicine Siriraj Hospital, Mahidol University, Office for Research and Development, Bangkok, Thailand, ² Thailand Ministry of Public Health-U.S. CDC Collaboration, Nonthaburi, Thailand, ³ Global AIDS Program, Centers for Disease Control and Prevention, Atlanta, GA, United States

BACKGROUND: The enumeration of CD4+ T cells is important in management of HIV-infected patients. However, the high cost of CD4+ T cell enumeration by current gold standard bead-based tests using a flow cytometer (FCM) makes them impractical in developing countries. Low-cost alternatives are needed. This study evaluated two recently developed inexpensive volumetric FCM techniques from Partec CyFlow and Guava Technologies.

METHODS: CD4+ T cell counts on whole blood samples from 250 HIV-1 infected Thai patients were determined in parallel by four methods: the new single-platform (SP) volumetric methods, CyFlowgreen FCM and Guava Personal Cell Analysis (PCA) system; and two standard SP bead-based methods, TriTEST/TruCOUNT™ tube using a FACSCalibur™ FCM and two-color FACSCount™ system. Correlation and agreement were analyzed using linear regression and Bland-Altman analysis.

RESULTS: When compared with the standard TriTEST/TruCOUNT™ and FACSCount™, the CD4+ T cell counts obtained from CyFlowgreen showed excellent correlation (R²=0.97 and 0.97, respectively, P<0.0001 for each); mean biases were –36.2 cells/μL (limit of agreement [LOA]: –149.7 to +77.3 cells/μL) and –16.1 cells/μL (LOA: –113.9 to +81.6 cells/μL), respectively. For the Guava PCA system compared with standard TriTEST/TruCOUNT™ and FACSCount™, correlation coefficients were high (R²=0.95 and 0.94, respectively, P<0.0001 for each) with mean biases of +13.1 cells/μL (LOA: –117.9 to +144.1 cells/μL) and +33.2 cells/μL (LOA: –101.8 to +168.3 cells/μL), respectively. Correlation of CD4+ T cell counts between the two volumetric SP methods was also high: R²=0.93, (P<0.0001) with mean bias of +49.4 cells/μL (LOA: –107.6 to +206.3 cells/μL).

CONCLUSIONS: The volumetric CyFlowgreen FCM and Guava PCA system performed well relative to the two standard bead-based systems. Use of these new technologies could make CD4+ T cell enumeration more affordable in Thailand and other resourcepoor settings.

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2006-08-13
TuAb0205

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