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4th International AIDS Society Conference on HIV Pathogenesis and TreatmentSydney, Australia - July 22 - 25, 2007 |
HIV-1 GENETIC DIVERSITY: A COMPARISON OF VIRAL EVOLUTION IN BLOOD AND THE VAGINAL TRACT RIGHT FROM SEROCONVERSION AND DURING DISEASE PROGRESSION
IAS Conf HIV Pathog Treat 2007 Jul 22-25;4th: Abstract No. MOAA201
Nankya I.1, Demers K.2, Kyeyune F.2, Bulime S.2, Nanyonjo H.2, Salata R.3, Arts E.3
1Case Western Reserve University, Microbiology and Molecualr Biology, Cleveland, United States, 2Joint Clinical Research Center, Virology, Kampala, Uganda, 3Division of Infectious Diseases, School of Medicine, Case Western Reserve University, Cleveland, United States
OBJECTIVES: We have previously shown that in the absence of treatment HIV-1 fitness increases with disease progression as indicated by significant correlations with increasing viral load, decreasing CD4 counts, and increasing HIV-1 genetic diversity. We have also shown that subtype C viruses are much less fit than any other group M. In this study we have compared the rate of viral evolution of subtype C to that of subtype A and D from two different cohorts and have shown that very early in infection, there is no difference in diversity, however, as the disease progresses, these differences may become more pronounced.
METHODS: Using a cohort of HIV-1 subtypes A- and D-infected Ugandan and subtype C- infected Zimbabwean women, we measured viral diversity in blood and vaginal tract at the time of seroconversion and at different time points during disease progression. Diversity in the HIV-1 V3 and V1/V2 regions was analyzed using heteroduplex tracking assay.
RESULTS: Within blood, a low viral diversity at seroconversion increases during disease progression. In contrast, a higher HIV-1 genetic diversity is observed in the vaginal tract as compared to blood after seroconversion, which then decreases in the first year to reach a set point and then increases as in blood. This pattern was the same in subtype A, C, and D infections regardless of whether the V3 or V1/V2 was employed for diversity analyses. We did however find that the rate of viral evolution was significantly higher in the V1/V2 region than in the V3. Also evolution was much slower in subtype C than A and D.
CONCLUSIONS: Preliminary results suggest that HIV-1 diversity is higher in vaginal tract than in blood at seroconversion but that the former undergoes a genetic bottleneck whereas the viral population in blood increases in genetic diversity.
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2007-07-22
MOAA201
HIV Diversity, Tropism and Compartmentalization
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