[O-17] High HIV viral load inhibits osteoblast function and signalling

10th International Workshop on Adverse Drug Reactions and Lipodystrophy in HIV

6-8 November 2008, London, UK

HIGH HIV VIRAL LOAD INHIBITS OSTEOBLAST FUNCTION AND SIGNALLING

Antiviral Therapy 2008; 13(Suppl. 4):A13 (abstract no. O-17)

N Chew^1,2,3, E Cotter¹, P Doran^1,3 and W Powderly^1,2,3
¹Catherine McCauley Research Centre, University College Dublin, Dublin, Ireland; ²Mater Misericordiae University Hospital, Dublin, Ireland; ³School of Medicine and Medical Sciences, University College Dublin, Dublin, Ireland

INTRODUCTION: The prevalence of osteoporosis in a HIV- positive cohort is more than three times higher than in matched HIV-negative controls. Although antiretroviral therapy (ART) treatment has been associated with increased odds of reduced bone density compared with ART-naïve patients, the pathogenic mechanisms underlying the initiation and progression of osteoporosis in HIV patients remain to be elucidated. Recent studies have reported altered bone biology and function in response to ART exposure, including effects on osteoclasts and osteoblasts. However, the direct effect of HIV on bone cell biology has not been evaluated.

Hypothesis: We hypothesized that exposure to HIV alters human osteoblast function and activity, and ultimately leads to osteopenia/osteoporosis.

METHODS: Primary human osteoblasts (hOB) were cultured in growth medium in vitro. Growth medium was supplemented with serum from three distinct patient groups (HIV-negative, HIV-positive low [viral load range 120–4,000] or high [viral load range 100,000–500,000] viral load serum [5% concentration, 72 h, n=5 per patient group, HIV serum obtained from ART-naïve patients]). Cell proliferation (as a biological endpoint) and calcium deposition (as a functional endpoint) were determined using established methods. In addition, to identify the effect of HIV on transcriptional regulators of the bone phenotype, real-time PCR with gene-specific primers was used to quantify mRNA expression of RUNX-2, a pro-osteogenic transcription factor.

RESULTS: Exposure of hOB to control low or high HIV viral load serum did not affect hOB cell proliferation, demonstrating that these exposures did not have a cytotoxic effect on osteoblasts in vitro. hOB calcium deposition reduced significantly (P<0.005) after treatment with high viral load serum compared with either low viral load or HIV-negative control serum. Osteoblast RUNX-2 mRNA expression declined by 25% (P<0.05) after exposure to high viral load serum compared with HIV-negative controls.

CONCLUSIONS: These data demonstrate bioactivity of HIV in the setting of osteoblast cell culture. Serum obtained from HIV patients with high viral load effected significant changes in the bone phenotype, as evidenced by reduced capacity for calcium deposition. Intriguingly, this functional effect was mirrored by changes in the expression of the osteogenic transcription factor RUNX-2. These findings support the hypothesis that HIV itself, in addition to the well described effect of ART, can modulate bone phenotype and, at least in part, drive the osteopenia and osteoporosis, which is increasingly seen in HIV patients.

2008-11-06
O-17

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