Your Search for amplification assay matched 183 Documents (Page 1 of 7).
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Abstract: The safety of the blood supply in the United States and elsewhere has been improved in recent years by the implementation of assays that detect HIV-1 antigen during that transient period of time in the infection process when viral titer is high. It is generally accepted that use of nuc ...
http://www.aegis.org/aidsline/2000/may/a0050177.html
File Name: a0050177.html, Size: 10KB, Last Modified: Sunday, May 28, 2000 at 8:12:04 PM
Abstract: We have used a branched DNA (bDNA) signal amplification assay for the direct detection of Mycobacterium tuberculosis DNA in alkaline-treated sputum specimens. The assay relies upon signal amplification using bDNA multimers and a chemiluminescent detection system. Extensive sample preparation is not required. The bacte ...
http://www.aegis.org/aidsline/1995/dec/m95c1838.html
File Name: m95c1838.html, Size: 9KB, Last Modified: Thursday, December 28, 1995 at 8:12:04 PM
Abstract: <p><b>OBJECTIVE</b>: To quantitate the amount of HIV RNA in patient plasma and correlate copy number with CD4 count and antiretroviral (ARV) therapy as measured by branched DNA (bDNA) signal amplification assay. METHODOLOGY: 1 ml of patient plasma was centrifuged in duplicate using a benchtop microfuge. The resulting ...
http://www.aegis.org/conferences/iac/1993/poa320795.html
File Name: poa320795.html, Size: 5KB, Last Modified: Sunday, June 06, 1993 at 8:12:04 PM
Abstract: The new assay was highly specific and demonstrated an increased sensitivity with a broader linear dynamic range compared to other amplification assays for HIV-1 RNA. ...
http://www.aegis.org/conferences/iashivpt/2001/403.html
File Name: 403.html, Size: 7KB, Last Modified: Monday, July 09, 2001 at 6:29:23 AM
Abstract: <p><b>BACKGROUND:</b> To investigate and describe the kinetics in HIV RNA Copies among cases with AIDS before and during the Chinese Herbs XQ-9302 treatment to evaluate the effects in AIDS treatment.</p> <p><b>METHODS:</b> Within the period of 1996-1999, based on the national criteria for AIDS diagnosis, a total of ...
http://www.aegis.org/conferences/iac/2000/thpeb5192.html
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Abstract: Background: Tuberculosis in HIV-infected patients is highly prevalent in Southern Europe. Rapid diagnosis is important to start specific therapy in these subjects. Objective: To study the clinical usefulness of the amplification assay in the detection of mycobacterium tuberculosis directly in clinical samples of patie ...
http://www.aegis.org/aidsline/1997/jan/m9712620.html
File Name: m9712620.html, Size: 9KB, Last Modified: Tuesday, January 28, 1997 at 8:12:04 PM
Abstract: Gen-Probe amplified mycobacterium tuberculosis direct test has the ability to reduce the time required for diagnosis of tuberculosis to one day. Compared to conventional methods, MTD test is a sensitive and specific procedure for the identification of mycobacterium tuberculosis. Rapid and specific therapy in patients i ...
http://www.aegis.org/conferences/hiv-glasgow/1996/p117.html
File Name: p117.html, Size: 7KB, Last Modified: Sunday, November 03, 1996 at 8:12:04 PM
Abstract: Background: Tuberculosis in HIV-infected patients is highly prevalent in Southern Europe. Rapid diagnosis is important to start specific therapy in these subjects. <p><b>OBJECTIVE</b>: To study the clinical usefulness of the amplification assay in the detection of mycobacterium tuberculosis directly in clinical sampl ...
http://www.aegis.org/conferences/iac/1996/tha4074.html
File Name: tha4074.html, Size: 6KB, Last Modified: Sunday, July 07, 1996 at 8:12:04 PM
Abstract: Objective: Evaluation of changes in quantitation of CMV DNA levels in serial leukocyte specimens. Methods: Cell pellets containing 10(5)-10(6) cells were assayed directly for CMV DNA using a bDNA signal amplification assay. Culture results were also available on ...
http://www.aegis.org/aidsline/1995/dec/m95c2826.html
File Name: m95c2826.html, Size: 9KB, Last Modified: Thursday, December 28, 1995 at 8:12:04 PM
Abstract: <p><b>OBJECTIVE</b>: To evaluate the feasibility of quantitating HIV RNA in Peripheral Blood Mononuclear Cells (PBMCs), and examine the relationship between HIV viral load in these cells with viral load in plasma using a bDNA signal amplification assay.</p> <p><b>METHODS</b>: Plasma and cell samples were obtained fr ...
http://www.aegis.org/conferences/iac/1994/580a.html
File Name: 580a.html, Size: 6KB, Last Modified: Thursday, August 04, 1994 at 8:12:04 PM
Abstract: <p><b>OBJECTIVE</b>: To assess HIV-1 RNA levels in plasma by branched DNA (bDNA) assay in patients receiving long-term Zidovudine ( AZT ) therapy and in a placebo group.</p> <p><b>METHODS</b>: 36 initially asymptomatic HIV infected patients enrolled in a controlled trial receiving AZT 1g/day (n = 17) or placebo (n = ...
http://www.aegis.org/conferences/iac/1994/577a.html
File Name: 577a.html, Size: 6KB, Last Modified: Thursday, August 04, 1994 at 8:12:04 PM
Abstract: AIM: To determine the relative effect of sample matrix on the quantitation of HIV RNA in plasma. METHOD: Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction were us ...
http://www.aegis.org/aidsline/1994/jul/m9470250.html
File Name: m9470250.html, Size: 9KB, Last Modified: Thursday, July 28, 1994 at 8:12:04 PM
Abstract: OBJECTIVE: To quantitate the amount of HIV RNA in patient plasma and correlate copy number with CD4 count and antiretroviral (ARV) therapy as measured by branched DNA (bDNA) signal amplification assay. METHODOLOGY: 1 ml of patient plasma was centrifuged in duplicate using a benchtop microfuge. The resulting virion pel ...
http://www.aegis.org/aidsline/1993/nov/m93b4909.html
File Name: m93b4909.html, Size: 9KB, Last Modified: Sunday, November 28, 1993 at 8:12:04 PM
Abstract: Measuring viral load in plasma is important for understanding AIDS pathogenesis and monitoring treatment efficacy. We compared a novel amplification assay using bDNA technology vs quantitative culture, p24 Ag and RT-PCR for detecting and quantifying plasma viremia. Our results to date showed that the bDNA assay has exc ...
http://www.aegis.org/conferences/iac/1993/poa320794.html
File Name: poa320794.html, Size: 5KB, Last Modified: Sunday, June 06, 1993 at 8:12:04 PM
Abstract: <p><b>OBJECTIVE</b>: To compare HIV-1 RNA levels in 133 plasma samples by the bDNA assay, RT-PCR, and plasma cultures. <p><b>METHODS</b>: Plasma was obtained from 13 patients sequentially over a period of 14-19 weeks, in a blinded AZT + immune modulator study. Frozen specimens were thawed prior to testing with all ...
http://www.aegis.org/conferences/iac/1993/pob412480.html
File Name: pob412480.html, Size: 6KB, Last Modified: Sunday, June 06, 1993 at 8:12:04 PM
Abstract: To determine the relative effect of the sample matrix from different patients on the quantitation of HIV RNA in plasma, HIV-positive plasma collected in EDTA was diluted into ten different HIV-negative plasma samples. Sample dilutions in the linear range were extracted individually, and RT-PCR was performed using the S ...
http://www.aegis.org/conferences/iac/1993/pob412483.html
File Name: pob412483.html, Size: 6KB, Last Modified: Sunday, June 06, 1993 at 8:12:04 PM
Abstract: The cell-associated viral burden (DNA and RNA) in HIV-infected elite controllers is strongly associated with level of potential target cells (activated CD4+ T-cells and HIV-specific CD4+ T-cells), but not with other host responses. Plasma HIV RNA levels were only associated with the %activated CD8+ T-cells. Collectivel ...
http://www.aegis.org/conferences/hivdrw/2009/5.html
File Name: 5.html, Size: 7KB, Last Modified: Tuesday, June 09, 2009 at 7:12:04 PM
Abstract: BACKGROUND: HIV-1 is characterized by extensive genetic diversity. Within group M, there are 9 subtypes (A-D, F-H, K) and 15 circulating recombinant forms (CRF). In Western Europe, subtype B predominates, but increasing numbers of non-B infections are being observed. In many countries, >20% of new infections are non-su ...
http://www.aegis.org/conferences/iac/2004/mopec3419.html
File Name: mopec3419.html, Size: 6KB, Last Modified: Monday, May 11, 2009 at 10:19:32 PM
Abstract: BACKGROUND: Human Immunodeficiency Virus-1 (HIV-1) viral load measurement is critical for monitoring disease progression and response to anti-retroviral therapy. The authors have developed a highly sensitive automated kinetic amplification assay that quantifies HIV-1 RNA in human plasma from all group M and O subtypes. ...
http://www.aegis.org/conferences/iac/2004/mopeb3167.html
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Abstract: Among HIV patients, lower CD4 counts, higher HIV viral loads, and the absence of treatment with an anti-HBV ART agent were associated with occult HBV. The prevalence of occult HBV was not greater in HIV+/HCV+ patients compared with HIV+/HCV– patients. The presence of anti-HBs does not rule out occult HBV infection. Tes ...
http://www.aegis.org/conferences/croi/2006/836.html
File Name: 836.html, Size: 7KB, Last Modified: Sunday, February 05, 2006 at 8:12:04 PM
Abstract: These preliminary results strongly suggest that the TB2 assay yields excellent predictive positive and negative values for diagnosis of tuberculosis encephalitis. More over, it is also suggested by the accuracy shown by these results that they might be helpful to exclude TB from infections caused by non-tuberculosis pa ...
http://www.aegis.org/conferences/iashivpt/2005/tupe7-1c21.html
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Abstract: BACKGROUND: Diagnostic test based on amplification and detection of nucleic acids of MTB directly in clinical samples, would yield results in much shorter time than the conventional techniques, allowing sooner treatments of the MTB-caused life-threatening encephalitis. We report preliminary results using a DNA-amplific ...
http://www.aegis.org/conferences/iac/2004/mopeb3183.html
File Name: mopeb3183.html, Size: 7KB, Last Modified: Monday, July 12, 2004 at 6:29:23 AM
Abstract: Background;The diagnosis of HIV-1 infection in neonates born to sero positive mothers presents two major dilemmas; the presence of maternal antibodies that preclude the use of serological tests, and the difficulty or impossibility of obtaining large volumes of blood for culture. Objectives;To evaluate a reliable method ...
http://www.aegis.org/conferences/iac/2004/b10283.html
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Abstract: BACKGROUND: Mitochondrial toxicity is associated with nucleoside analogues (NRTIs) and may contribute to lipodystrophy. We studied mitochondrial DNA (mtDNA) and RNA (mtRNA) levels in patients switched to this NRTI sparing regimen after failing NRTIs. Significant increases in limb and visceral fat were previously report ...
http://www.aegis.org/conferences/iac/2004/weppb2064.html
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Abstract: BACKGROUND: As the prevalence of HIV-1 subtypes and recombinants increases throughout the world, accurate viral load quantification and detection will be paramount for effective management of HIV+ individuals. To this end, a real-time NASBA amplification assay was developed for measurement of HIV-1 RNA levels in plasma ...
http://www.aegis.org/conferences/croi/2004/960.html
File Name: 960.html, Size: 7KB, Last Modified: Sunday, February 08, 2004 at 8:12:04 PM
Abstract: These preliminary results strongly suggest that the TB2 assay yield excellent predictive positive and negative values for diagnosis of tuberculosis encephalitis. More over, it is also suggested by the accuracy shown by these results that they might be helpful to exclude M. tuberculosis from infections caused by NTP. Fi ...
http://www.aegis.org/conferences/eacs/2001/posters/107.html
File Name: 107.html, Size: 8KB, Last Modified: Monday, December 22, 2003 at 7:09:52 PM
Abstract: AEGIS, 8th EUROPEAN CONFERENCE ON CLINIC ASPECTS AND TREATMENT OF HIV - INFECTION. Abstract Form. REFERENCE NUMBER : 155. ECCATH ID : P327. 8th EUROPEAN CONFERENCE ON CLINIC ASPECTS AND TREATMENT OF HIV - INFECTION. Location of research or project (country). Argentina. Thematic Areas:. 8.3. DETECTION OF MICOBACTERIU ...
http://www.aegis.org/conferences/eacs/2001/papers/107.html
File Name: 107.html, Size: 6KB, Last Modified: Sunday, November 30, 2003 at 1:18:16 AM
Abstract: The NucliSens EasyQ HIV-1 is a highthroughput real-time viral load assay that demonstrates broad subtype reactivity and good correlation with two FDA-approved HIV-1 viral load assays. ...
http://www.aegis.org/conferences/iashivpt/2003/1232.html
File Name: 1232.html, Size: 7KB, Last Modified: Monday, July 14, 2003 at 6:29:23 AM
Abstract: In the first 6 months of life, IU and IP/ePP transmission contributed about 25% of the 30.7% MTCT. This is the first study with a large cohort, in the absence of antiretroviral intervention, to define contributions of the three modes of MTCT. Our data, in addition to serving as a historical comparison, may be useful in ...
http://www.aegis.org/conferences/iashivpt/2003/58.html
File Name: 58.html, Size: 7KB, Last Modified: Monday, July 14, 2003 at 6:29:23 AM
Abstract: Analysis of a set of 223 randomly selected clinical serum samples revealed that NucliSens EasyQ HIV-1 has a comparable performance the reference method NucliSens HIV-1 QT. Samples not quantifiable by either one of the assays are in most cases in the low viral load range. Regarding the workflow it is found that NucliSen ...
http://www.aegis.org/conferences/iashivpt/2003/678.html
File Name: 678.html, Size: 7KB, Last Modified: Monday, July 14, 2003 at 6:29:23 AM